222550

Nile Blue chloride

Dye content 85 %

• Empirical Formula (Hill Notation): C₂₀H₂₀ClN₃O
• CAS Number: 2381-85-3
• Molecular Weight: 353.85
• Colour Index Number: 51180
• Beilstein: 3923952
• MDL number: MFCD00011936
• UNSPSC Code: 12171500
• PubChem Substance ID: 24853342
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 200
• composition: Dye content, 85%
• solubility: water: 1 mg/mL, opaque, blue to very deep blue
• λmax: 638 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34
• InChI: 1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H
• InChI key: SHXOKQKTZJXHHR-UHFFFAOYSA-N

Description

Application

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Biochem/physiol Actions

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves