MAB3136
Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5
clone 4F5, Chemicon®, from mouse
Synonym(s):
NHE3
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4F5, monoclonal
species reactivity
rat, rabbit
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rabbit ... Slc9A3(100009430)
rat ... Slc9A3(24784)
Specificity
Na+/H+ exchanger, isoform NHE3.
Immunogen
Epitope: isoform NHE3
Maltose Binding Protein fusion protein containing C-terminal 131 amino acids of rabbit NHE3.
Application
Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5 detects level of Na+/H+ Exchanger-3 & has been published & validated for use in IC, IH(P), IP & WB.
Immunoblotting (rabbit 80 kDa and rat ~83 kDa)
Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.
Immunocytochemistry
Immunoprecipitation (use non-ionic detergent)
Optimal working dilutions must be determined by the user.
Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.
Immunocytochemistry
Immunoprecipitation (use non-ionic detergent)
Optimal working dilutions must be determined by the user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Linkage
Replaces: MAB3134
Physical form
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yanling Yan et al.
Molecular and cellular biochemistry, 367(1-2), 175-183 (2012-05-24)
We have demonstrated that ouabain regulates protein trafficking of the Na/K-ATPase α1 subunit and NHE3 (Na/H exchanger, isoform 3) via ouabain-activated Na/K-ATPase signaling in porcine LLC-PK1 cells. To investigate whether this mechanism is species-specific, ouabain-induced regulation of the α1 subunit
Elevated BSC-1 and ROMK expression in Dahl salt-sensitive rat kidneys.
Hoagland, KM; Flasch, AK; Dahly-Vernon, AJ; dos Santos, EA; Knepper, MA; Roman, RJ
Hypertension null
Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells.
Li, XC; Hopfer, U; Zhuo, JL
American Journal of Physiology: Renal Physiology null
Xiaochun Li et al.
Cells, 12(11) (2023-06-10)
The current prevailing paradigm in the renin-angiotensin system dictates that most, if not all, biological, physiological, and pathological responses to its most potent peptide, angiotensin II (Ang II), are mediated by extracellular Ang II activating its cell surface receptors. Whether
Brandon A Kemp et al.
Journal of the American Heart Association, 8(9), e012016-e012016 (2019-05-02)
Background Previous studies demonstrated that angiotensin (Ang) III , not Ang II , is the predominant endogenous agonist for Ang type-2 receptor ( AT 2R)-induced natriuresis in normal rats, and that hypertensive 12-week-old spontaneously hypertensive rats ( SHR ) lack
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