07-741
Anti-AS160 (Rab-GAP) Antibody
serum, Upstate®
Synonym(s):
Acrg embryonic lethality minimal region ortholog, Akt substrate of 160 kDa, TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family, member
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
canine, human, mouse, rat
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TBC1D4(9882)
mouse ... Tbc1D4(210789)
General description
AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.
Specificity
Predicted to cross react with rat and dog based on sequence homology.
Recognizes AS160 (Rab-GAP), Mr 160 kDa.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kDa (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes.
Application
Detect AS160 (Rab-GAP) using this Anti-AS160 (Rab-GAP) Antibody validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Quality
Routinely evaluated by Western Blot in RIPA lysates from 3T3/L1 cells differentiated into adipocytes.
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. This lot also detects AS160 (Rab-GAP) in HEK293 lysates.
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. This lot also detects AS160 (Rab-GAP) in HEK293 lysates.
Target description
Approx. 160 kDa
Physical form
Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
MDI-treated 3T3/L1 cell lysates.
MDI-treated 3T3/L1 cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Clustering of GLUT4, TUG, and RUVBL2 protein levels correlate with myosin heavy chain isoform pattern in skeletal muscles, but AS160 and TBC1D1 levels do not.
Castorena, CM; Mackrell, JG; Bogan, JS; Kanzaki, M; Cartee, GD
Journal of Applied Physiology (1985)
AS160 phosphorylation is associated with activation of alpha2beta2gamma1- but not alpha2beta2gamma3-AMPK trimeric complex in skeletal muscle during exercise in humans.
Treebak, JT; Birk, JB; Rose, AJ; Kiens, B; Richter, EA; Wojtaszewski, JF
American Journal of Physiology. Endocrinology and Metabolism null
Joanne Bruno et al.
The Journal of cell biology, 214(1), 61-76 (2016-06-30)
RAB10 is a regulator of insulin-stimulated translocation of the GLUT4 glucose transporter to the plasma membrane (PM) of adipocytes, which is essential for whole-body glucose homeostasis. We establish SEC16A as a novel RAB10 effector in this process. Colocalization of SEC16A
Myricetin Ameliorates Defective Post-Receptor Insulin Signaling via ?-Endorphin Signaling in the Skeletal Muscles of Fructose-Fed Rats.
Tzeng, TF; Liou, SS; Liu, IM
Evidence-Based Complementary and Alternative Medicine : ECAM null
Chao Quan et al.
PloS one, 10(4), e0124491-e0124491 (2015-04-30)
The Rab GTPase activating protein (RabGAP), AS160/TBC1D4, is an important substrate of protein kinase B (PKB), and regulates insulin-stimulated trafficking of glucose transporter 4. Besides, AS160/TBC1D4 has also been shown to regulate trafficking of many other membrane proteins including FA
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