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  • c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility.

c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility.

eLife (2013-12-19)
Bridget R Kulasekara, Cassandra Kamischke, Hemantha D Kulasekara, Matthias Christen, Paul A Wiggins, Samuel I Miller
要旨

Individual cell heterogeneity is commonly observed within populations, although its molecular basis is largely unknown. Previously, using FRET-based microscopy, we observed heterogeneity in cellular c-di-GMP levels. In this study, we show that c-di-GMP heterogeneity in Pseudomonas aeruginosa is promoted by a specific phosphodiesterase partitioned after cell division. We found that subcellular localization and reduction of c-di-GMP levels by this phosphodiesterase is dependent on the histidine kinase component of the chemotaxis machinery, CheA, and its phosphorylation state. Therefore, individual cell heterogeneity in c-di-GMP concentrations is regulated by the activity and the asymmetrical inheritance of the chemotaxis organelle after cell division. c-di-GMP heterogeneity results in a diversity of motility behaviors. The generation of diverse intracellular concentrations of c-di-GMP by asymmetric partitioning is likely important to the success and survival of bacterial populations within the environment by allowing a variety of motility behaviors. DOI: http://dx.doi.org/10.7554/eLife.01402.001.

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Sigma-Aldrich
抗VSV-G抗体 ウサギ宿主抗体, affinity isolated antibody, buffered aqueous solution
Millipore
モノクローナル抗VSV糖タンパク質抗体-アガロース マウス宿主抗体, clone P5D4, purified from hybridoma cell culture, PBS suspension