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  • Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions.

Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions.

Electrophoresis (1995-03-01)
G Sreeramulu, N K Singh
要旨

A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (< 0.1 M) salt solutions were unsuitable. The method affords the advantage that expensive organic solvents, such as methanol, acetic acid or trichloroacetic acid, are not needed. Furthermore, salt destaining results in darker purple-blue protein bands as compared to the pale blue color with e.g. methanol/acetic acid destaining.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
ブリリアントブル-R, 250, for microscopy
Sigma-Aldrich
ブリリアントブル-R, pure
Sigma-Aldrich
ブリリアントブル-R, Dye content ~50 %, Technical grade
Sigma-Aldrich
ブリリアントブルーR染色液, suitable for (for immunoelectrophoresis protein staining)
Sigma-Aldrich
ブリリアントブルーR、濃縮液, suitable for SDS-PAGE, methanol solution