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  • A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis.

A modified coomassie brilliant blue G 250 staining method for the detection of chitinase activity and molecular weight after polyacrylamide gel electrophoresis.

Journal of bioscience and bioengineering (2008-08-12)
Chun Yi Liau, Chung-Sheng Lin
要旨

A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated that our method is more sensitive and less complicated than the conventional Calcofluor white M2R staining.

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製品番号
ブランド
製品内容

Sigma-Aldrich
ブリリアントブル-R, 250, for microscopy
Sigma-Aldrich
ブリリアントブル-R, pure
Sigma-Aldrich
ブリリアントブル-R, Dye content ~50 %, Technical grade
Sigma-Aldrich
ブリリアントブルーR、濃縮液, suitable for SDS-PAGE, methanol solution
Sigma-Aldrich
ブリリアントブルーR染色液, suitable for (for immunoelectrophoresis protein staining)