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Merck
  • Involvement of the arachidonic acid cascade in the hypersusceptibility to pentylenetetrazole-induced seizure during diazepam withdrawal.

Involvement of the arachidonic acid cascade in the hypersusceptibility to pentylenetetrazole-induced seizure during diazepam withdrawal.

Biological & pharmaceutical bulletin (2012-12-05)
Tomohisa Mori, Norifumi Shimizu, Masahiro Shibasaki, Tsutomu Suzuki
要旨

The present study was designed to clarify whether the arachidonic acid cascade contributes to the decreased threshold for pentylenetetrazole-induced seizure under benzodiazepine withdrawal in mice. The seizure threshold for pentylenetetrazole was significantly decreased by the discontinuation of chronic treatment with diazepam. The decrease in the seizure threshold for pentylenetetrazole during diazepam withdrawal was significantly suppressed by intracerebroventricular (i.c.v.) pretreatment with the phospholipase A(2) inhibitor quinacrine (30, 100 nmol) and the lipoxygenase inhibitor nordihydroguaiaretic acid (10, 30 nmol). In contrast, the decreased seizure threshold in the diazepam-withdrawal group was intensified by pretreatment with the cyclooxygenase inhibitor diclofenac (56 nmol). These compounds did not alter the threshold for seizure in a control group. These findings suggest that enhancement of the arachidonic acid cascade may contribute to the hypersusceptibility to pentylenetetrazole-induced seizure during diazepam withdrawal.

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Sigma-Aldrich
ペンチレンテトラゾール
Sigma-Aldrich
リポキシダーゼ Glycine max(ダイズ)由来, Type I-B, lyophilized powder, ≥50,000 units/mg solid
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キナクリン 二塩酸塩, ≥90%
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リポキシダーゼ Glycine max(ダイズ)由来, Type V, ammonium sulfate suspension, 500,000-1,000,000 units/mg protein
Sigma-Aldrich
ノルジヒドログアイアレチン酸, ≥97.0% (HPLC)