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  • Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.

Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.

Cell stem cell (2022-09-03)
Yi Zheng, Robin Zhexuan Yan, Shiyu Sun, Mutsumi Kobayashi, Lifeng Xiang, Ran Yang, Alexander Goedel, Yu Kang, Xufeng Xue, Sajedeh Nasr Esfahani, Yue Liu, Agnes M Resto Irizarry, Weisheng Wu, Yunxiu Li, Weizhi Ji, Yuyu Niu, Kenneth R Chien, Tianqing Li, Toshihiro Shioda, Jianping Fu
要旨

Despite its clinical and fundamental importance, our understanding of early human development remains limited. Stem cell-derived, embryo-like structures (or embryoids) allowing studies of early development without using natural embryos can potentially help fill the knowledge gap of human development. Herein, transcriptome at the single-cell level of a human embryoid model was profiled at different time points. Molecular maps of lineage diversifications from the pluripotent human epiblast toward the amniotic ectoderm, primitive streak/mesoderm, and primordial germ cells were constructed and compared with in vivo primate data. The comparative transcriptome analyses reveal a critical role of NODAL signaling in human mesoderm and primordial germ cell specification, which is further functionally validated. Through comparative transcriptome analyses and validations with human blastocysts and in vitro cultured cynomolgus embryos, we further proposed stringent criteria for distinguishing between human blastocyst trophectoderm and early amniotic ectoderm cells.

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製品内容

Sigma-Aldrich
抗GAPDH抗体、マウスモノクローナル, clone GAPDH-71.1, purified from hybridoma cell culture
Sigma-Aldrich
抗Sox2抗体, Chemicon®, from rabbit
Sigma-Aldrich
Mouse Anti-Stella Antibody, clone 3H5.2, Chemicon®, from mouse