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Merck

Generation of Mammalian offspring by haploid embryonic stem cells microinjection.

Current protocols in stem cell biology (2014-11-05)
Ling Shuai, Wei Li, Haifeng Wan, Xiao-Yang Zhao, Liu Wang, Qi Zhou
要旨

In this unit we introduce the derivation and genetic modification of mouse haploid embryonic stem (ES) cells. We detail how to produce haploid embryos and the subsequent ES derivation and cell culture. We further introduce readers to the intracytoplasmic injection processes of two types of haploid ES cells [androgenetic haploid ES (ahES) and parthenogenetic ES (phES)], both of which possess potential to produce fertile progenies by microinjection. This unit will be interesting to researchers who focus on recessive screens and transgenic animal model production with haploid stem cells.

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製品番号
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製品内容

Sigma-Aldrich
ESGRO®リコンビナントマウスLIFタンパク質, ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.
Roche
インスリン、ヒト, recombinant (yeast)
Sigma-Aldrich
ウシ血清アルブミン ウシ血清由来, powder, BioXtra
Sigma-Aldrich
マイトマイシンC Streptomyces caespitosus由来, powder, contains NaCl as solubilizer
Sigma-Aldrich
(±)-ベラパミル 塩酸塩, ≥99% (titration), powder
Sigma-Aldrich
絨毛性ゴナドトロピン ヒト, lyophilized powder, from human pregnancy urine