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Merck

Miro1 Impairment in a Parkinson's At-Risk Cohort.

Frontiers in molecular neuroscience (2021-08-27)
David Nguyen, Vinita Bharat, Devon M Conradson, Pawan Nandakishore, Xinnan Wang
要旨

There is a lack of reliable molecular markers for Parkinson's disease (PD) patients and at-risk individuals. The detection of the pre-symptomatic population of PD will empower more effective clinical intervention to delay or prevent disease onset. We have previously found that the mitochondrial protein Miro1 is resistant to mitochondrial depolarization-induced degradation in fibroblasts from a large number of PD patients and several at-risk individuals. Therefore, Miro1 has the potential to molecularly label PD populations. In order to determine whether Miro1 could serve as a molecular marker for the risk of PD, here we examine the Miro1 response to mitochondrial depolarization by biochemical approaches in induced pluripotent stem cells from a cohort of at-risk individuals. Our results show that the Miro1 phenotype is significantly associated with PD risk. We propose that Miro1 is a promising molecular marker for detecting both PD and at-risk populations. Tracking this Miro1 marker could aid in diagnosis and Miro1-based drug discoveries.

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製品内容

Millipore
Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
Sigma-Aldrich
カルボニルシアニド 3-クロロフェニルヒドラゾン, ≥97% (TLC), powder
Sigma-Aldrich
Monoclonal Anti-RHOT1 antibody produced in mouse, clone 4H4, purified immunoglobulin, buffered aqueous solution