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  • Two types of somatostatin-expressing GABAergic interneurons in the superficial layers of the mouse cingulate cortex.

Two types of somatostatin-expressing GABAergic interneurons in the superficial layers of the mouse cingulate cortex.

PloS one (2018-07-13)
Therese Riedemann, Tobias Straub, Bernd Sutor
要旨

Somatostatin-expressing (SOM+), inhibitory interneurons represent a heterogeneous group of cells and given their remarkable diversity, classification of SOM+ interneurons remains a challenging task. Electrophysiological, morphological and neurochemical classes of SOM+ interneurons have been proposed in the past but it remains unclear as to what extent these classes are congruent. We performed whole-cell patch-clamp recordings from 127 GFP-labeled SOM+ interneurons ('GIN') of the superficial cingulate cortex with subsequent biocytin-filling and immunocytochemical labeling. Principal component analysis followed by k-means clustering predicted two putative subtypes of SOM+ interneurons, which we designated as group I and group II GIN. A key finding of our study is the fact that these electrophysiologically and morphologically distinct groups of SOM+ interneurons can be correlated with two neurochemical subtypes of SOM+ interneurons described recently in our laboratory. In particular, all SOM+ interneurons expressing calbindin but no calretinin could be classified as group I GIN, whereas all but one neuropeptide Y- and calretinin-positive interneurons were found in group II.

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抗ソマトスタチン抗体 クローンYC7, culture supernatant, clone YC7, Chemicon®
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Anti-GFP (Green Fluorescent Protein) Antibody, Upstate®, from chicken
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Anti-Neuropeptide Y Antibody, serum, Chemicon®
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