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  • Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics.

Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics.

The Journal of cell biology (2018-03-15)
Coralie Fassier, Amélie Fréal, Laïla Gasmi, Christian Delphin, Daniel Ten Martin, Stéphanie De Gois, Monica Tambalo, Christophe Bosc, Philippe Mailly, Céline Revenu, Leticia Peris, Susanne Bolte, Sylvie Schneider-Maunoury, Corinne Houart, Fatiha Nothias, Jean-Christophe Larcher, Annie Andrieux, Jamilé Hazan
要旨

During neural circuit assembly, extrinsic signals are integrated into changes in growth cone (GC) cytoskeleton underlying axon guidance decisions. Microtubules (MTs) were shown to play an instructive role in GC steering. However, the numerous actors required for MT remodeling during axon navigation and their precise mode of action are far from being deciphered. Using loss- and gain-of-function analyses during zebrafish development, we identify in this study the meiotic clade adenosine triphosphatase Fidgetin-like 1 (Fignl1) as a key GC-enriched MT-interacting protein in motor circuit wiring and larval locomotion. We show that Fignl1 controls GC morphology and behavior at intermediate targets by regulating MT plus end dynamics and growth directionality. We further reveal that alternative translation of Fignl1 transcript is a sophisticated mechanism modulating MT dynamics: a full-length isoform regulates MT plus end-tracking protein binding at plus ends, whereas shorter isoforms promote their depolymerization beneath the cell cortex. Our study thus pinpoints Fignl1 as a multifaceted key player in MT remodeling underlying motor circuit connectivity.

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Sigma-Aldrich
抗アセチル化チューブリン抗体、マウスモノクローナル マウス宿主抗体, clone 6-11B-1, purified from hybridoma cell culture
Sigma-Aldrich
ノコダゾール, ≥99% (TLC), powder
Sigma-Aldrich
抗GABA ウサギ宿主抗体, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
抗αチューブリン抗体、マウスモノクローナル, clone B-5-1-2, purified from hybridoma cell culture
Sigma-Aldrich
モノクロナール抗チューブリン, チロシン マウス宿主抗体, clone TUB-1A2, ascites fluid