G006032

HPLC Analysis MAb Variants on Proteomix^® SCX-NP5, Fast Analysis

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777164); mobile phase: [A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5; gradient: top (0.8 mL/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 mL/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min; flow rate: 0.8 mL/min or 1.5 mL/min; detector: UV 280 nm; injection: 20 μL; sample: MAb 321, 5 mg/mL
• 適合性: application for HPLC
• アプリケーション: life science and biopharma

詳細

アナリシスノート

Due to the low pressure of Proteomix SCX, faster analysis and high resolution can be achieved with a higher flow rate.

法的情報

Proteomix is a registered trademark of Sepax Technologies