G005802

HPLC Analysis of Polar Compounds on Ascentis^® Express OH5 (HILIC Mode)

application for HPLC

特性

• テクニック: HPLC: suitable
• 試験パラメータ: column: Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 μm particles (53769-U); mobile phase: 5 mM ammonium formate (95:5 acetonitrile:water) pH 6.8; flow rate: 0.6 mL/min; pressure: 965 psi (66.5 bar); column temp.: 30 °C; detector: UV, 254 nm; injection: 1 μL; sample: 100 μg/mL in 25:75, water:methanol
• 適合性: application for HPLC
• アプリケーション: pharmaceutical (small molecule)

詳細

アナリシスノート

The impact of partitioning and ion exchange in HILIC separations is demonstrated using a mixture of acidic, neutral, and basic polar compounds in this Figure. All three analytes have similar log D values, both acid and base are ionized at mobile phase pH.

法的情報

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany