ZRB2775

Anti-CD163 Antibody, clone 1D3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: CD163, Hemoglobin scavenger receptor, Scavenger receptor cysteine-rich type 1 protein M130
• UNSPSCコード: 12352203

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1D3, recombinant monoclonal
• 詳細: recombinant, expressed in HEK 293 cells
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 125.45 kDa; observed mol wt ~150 kDa
• 精製方法: using Protein A
• 化学種の反応性: human
• 包装: antibody small pack of 25
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: N-terminal extracellular domain
• タンパク質IDアクセッション番号: NP_004235
• UniProtアクセッション番号: Q86VB7
• 環境により配慮した代替製品カテゴリ: Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• 遺伝子情報: human ... CD163(9332)

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1D3 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects CD163. It targets an epitope within 17 amino acids from the N-terminal extracellular domain.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal extracellular domain of human CD163.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD163 in human liver tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD163 in lysate from human spleen tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD163 in human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound CD163 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Scavenger receptor cysteine-rich type 1 protein M130 (UniProt: Q86VB7; also known as Hemoglobin scavenger receptor, CD163) is encoded by the CD163 (also known as M130) gene (Gene ID: 9332) in human. CD163 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains a long extracellular domain (aa 42-1050), a transmembrane domain (aa 1051-1071), and a cytoplasmic domain (aa 1072-1156). CD163 is expressed in monocytes and mature macrophages with high expression observed in bone marrow macrophages, Kupffer cells, lung macrophages, and red pulp macrophages. Its levels are up regulated by anti-inflammatory mediators and suppressed by pro-inflammatory mediators like lipopolysaccharides (LPS) and TNF. It serves as an acute phase-regulated receptor involved in clearance and endocytosis of Hb/Hp complexes by macrophages and protects tissues from free hemoglobin-mediated oxidative damage. The binding of the Hb/Hp complex to CD163 macrophages leads to a fast degradation of the complex. CD163 also plays a role in the uptake and recycling of iron, via endocytosis of Hb/Hp complex and subsequent breakdown of heme. A soluble form of CF163 (sCD163) has also been described that is produced by proteolytic shedding and can be induced by LPS, phorbol ester, and Fc region of immunoglobulin. This soluble form plays an anti-inflammatory role and is considered a diagnostic parameter for monitoring macrophage activation in inflammatory conditions. Its expression is markedly induced by anti- inflammatory mediators, such as glucocorticoids and interleukin 10. Clone MAC2-158 binds to SRCR domain 1 of CD163 and recognizes over 80% of circulating CD14-expressing monocytes. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhi, Y., et al. (2017). Mol. Med. Rep. 15(5); 2931-2939; Maniecki, MB., et al. (2011). Immunology 216(8); 882-890; Morganelli, PM., and Guyre, PM. (1988). J. Immunol. 140(7); 2296-2304).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

再構成

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable