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安全性情報

ZRB2155

Sigma-Aldrich

Anti-ROCK2 Antibody, clone 1B20 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

別名:

Rho-associated protein kinase 2;Rho kinase 2;Rho-associated coiled-coil-containing protein kinase 2;Rho-associated coiled-coil-containing protein kinase II;ROCK-II;p164 ROCK-2

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.43

由来生物

rabbit

品質水準

リコンビナント

expressed in HEK 293 cells

結合体

unconjugated

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

1B20, recombinant monoclonal

詳細

1B20 Clone

製品種目

ZooMAb® learn more

フォーム

lyophilized

分子量

calculated mol wt 160.9 kDa
observed mol wt ~165 kDa

精製方法

using Protein A

交差性

rat, human, mouse

交差性(ホモロジーによる予測)

monkey, canine, feline, horse

包装

antibody small pack of 25 μL

環境により配慮した代替製品の特徴

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

強化検証

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

テクニック

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

アイソタイプ

IgG

エピトープ配列

C-terminal

タンパク質IDアクセッション番号

UniProtアクセッション番号

環境により配慮した代替製品カテゴリ

輸送温度

ambient

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... ROCK2(9475)

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1B20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Rho-associated protein kinase 2 (ROCK2). It targets an epitope within 21 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Rho-associated protein kinase 2 (ROCK2).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ROCK2 in HepG2 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ROCK2 in A549, Raw264.7, and C6 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ROCK2 in human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ROCK2 in C6 cells.

Affinity Binding Assay: A representative lot of this antibody bound ROCK2 peptide with a KD of 1.8 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ROCK2 in HepG2 cell lysate.

ターゲットの説明

Rho-associated protein kinase 2 (UniProt: O75116; also known as EC:2.7.11.1, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, ROCK-II, p164 ROCK-2) is encoded by the ROCK2 (also known as KIAA0619) gene (Gene ID: 9475) in human. Rho kinase (ROCK), a serine/threonine kinase, serves as a target protein for small GTP-binding protein Rho and an important mediator of numerous cellular functions, including focal adhesions, motility, smooth muscle contraction, and cytokinesis. Rho kinase is found in two forms, ROCK 1 and ROCK2. They share 65% overall identity and 92% identity in the kinase domain. ROCK2 is mainly localized in the cytoplasm where it associates with actin microfilaments and the plasma membrane. It contains an amino-terminal catalytic kinase domain (aa 92-354) , a central coiled-coil domain of about 600 amino acids, and a carboxyl-terminal pleckstrin homology (PH) domain (aa 1150-1349) that is split by a cysteine-rich region. An interaction between its threonine 414 and aspartic acid 48 is shown to be essential for its kinase activity and dimerization. Phosphorylation at tyrosine 722 is reported to reduce its binding to RHOA and is crucial for focal adhesion dynamics. Since the ROCK-mediated pathway plays an important role in vascular smooth muscle contraction, cell adhesion and cell motility, it has gained importance in the pathogenesis of atherosclerosis. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hartmann, S., et al. (2015). Front. Pharmacol. 6; 276; Shi, J., et al. (2013). Cell Death Disease. 4; e483).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

11 - Combustible Solids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ZRB2155-4X25UL:
ZRB2155-25UL:


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