ZRB2141

Anti-OAS1 Antibody, clone 1N2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: 2′-5′-oligoadenylate synthase 1;EC:2.7.7.84;(2-5′)oligo(A) synthase 1;2-5A synthase 1;E18/E16;p46/p42 OAS
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1N2, recombinant monoclonal
• 詳細: 1N2 Clone
• 製品種目: ZooMAb^® learn more
• フォーム: lyophilized
• 分子量: calculated mol wt 46.03 kDa; observed mol wt ~52 kDa
• 精製方法: using Protein A
• 交差性: human
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_058132
• UniProtアクセッション番号: P00973
• 環境により配慮した代替製品カテゴリ: Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... OAS1(4938)

詳細

詳細

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1N2 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects 2′-5′-oligoadenylate synthase 1 (OAS1). It targets an epitope within 17 amino acids from the N-terminal half.

免疫原

KLH conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human 2′-5′-oligoadenylate synthase 1 (OAS1).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells treated with IFN- .

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected OAS1 in lysate from HeLa cells treated with IFN- .

Tested Applications

Enzyme Immunoassay (ELISA) Analysis: Various dilutions from a representative lot detected OAS1 in ELISA applicaiton.

Affinity Binding Assay: A representative lot of this antibody bound OAS1 peptide with a KD of 1.4 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected OAS1 in Human small intestine tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OAS1 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

2′-5′-oligoadenylate synthase 1 (UniProt: P00973; also known as EC:2.7.7.84, (2-5′)oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS) is encoded by the OAS1 (also known as OIAS) gene (Gene ID: 4938) in human. OAS is an interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. It is frequently detected in nasal epithelium of healthy individuals and displays antiviral activity. It is involved in the synthesis of higher oligomers of 2′-5′-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L then leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis and termination of viral replication. By activating RNase L, it potently inhibits severe acute SARS-CoV-2. Its C-terminal prenylation (isoform p46) is shown to be essential to initiate a SARS-CoV-2 block. The prenylated form is targeted to perinuclear structures rich in viral dsRNA, whereas the non-prenylated form (isoform p42) is diffusely localized and is unable to initiate a detectable block to SARS-CoV-2 replication. OAS1 also has antiviral effects that are independent of RNase L. OAS1 also plays a role in other cellular processes, including apoptosis, cell growth, differentiation, and gene regulation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wickenhagen, A. et al. (2021). Science. 374(6567); eabj3624).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable