ZRB1944

Anti-TIMP-1 Antibody, clone 1H19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: Metalloproteinase inhibitor 1;Erythroid-potentiating activity;EPA;Fibroblast collagenase inhibitor;Collagenase inhibitor;Tissue inhibitor of metalloproteinases 1
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1H19, recombinant monoclonal
• 詳細: 1H19 Clone
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 23.17 kDa; observed mol wt ~31 kDa
• 精製方法: using Protein A
• 化学種の反応性: human
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_003245
• UniProtアクセッション番号: P01033
• 環境により配慮した代替製品カテゴリ: Aligned,
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TIMP1(7076)

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1H19 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects TIMP-1. It targets an epitope within 15 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human TIMP-1.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TIMP-1 in MDA-MB-231 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TIMP-1 in HeLa cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound TIMP1 peptide with a KD of 1.2 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TIMP-1 in human prostate tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in MDA-MB-231 cell lysate

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TIMP-1 in MDA-MB-231 cell lysate.

ターゲットの説明

Metalloproteinase inhibitor 1 (UniProt: P01033; also known as Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, TIMP-1) is encoded by the TIMP1 (also known as CLGI, TIMP) gene (Gene ID: 7076) in human. The tissue inhibitors of metalloproteinases (TIMPs) represent a family of ubiquitous proteins which are natural inhibitors of the matrix metalloproteinases (MMPs). There are four known members of the TIMP family that are designated as TIMP-1, 2, 3, and 4. TIMPs are released during tissue damage to facilitate tissue remodeling through degradation and reorganization of the extracellular matrix. During this process MMPs are involved in inflammatory response through proteolytic maturation of cytokines. These activities are regulated through a 1:1 stoichiometric interaction of MMPs with TIMPs and these interactions are tightly controlled. TIMP-1 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein (aa 24-207). It is the best characterized inhibitor of MMP activity and regulates 14 of the 24 known MMPs. It inhibits MMP activity through the binding of its N-terminus with the targeted MMP, which results in chelation of Zn2+ from the enzyme active site. TIMP-1 levels are elevated in plasma of cancer subjects and its higher levels are associated with worse clinical outcomes. Stroma of human prostate and colon cancer is reported to express higher levels of TIMP-1 compared to their normal counterparts. This increased expression is reported to promotes tumor growth. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Knight, BE., et al. (2019). Front. Mol. Neurosci. 12; 220; Gong, Y., et al. (2013). PLoS ONE 8(10): e77366).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

再構成

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable