ZRB1920

Anti-Nanog Antibody, clone 4D17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 別名: Homeobox protein NANOG;ES cell-associated protein 4;Early embryo specific expression NK-type homeobox protein;Homeobox transcription factor Nanog
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• リコンビナント: expressed in HEK 293 cells
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 4D17, recombinant monoclonal
• 詳細: 4D17 Clone
• 製品種目: ZooMAb^® learn more
• 形状: lyophilized
• 分子量: calculated mol wt 31.86 kDa; observed mol wt ~37 and 32 kDa
• 精製方法: using Protein A
• 化学種の反応性: mouse
• 包装: antibody small pack of 25 μL
• 環境により配慮した代替製品の特徴: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: N-terminal half
• タンパク質IDアクセッション番号: NP_082292
• UniProtアクセッション番号: Q80Z64
• 環境により配慮した代替製品カテゴリ: , Aligned
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Nanog(71950)

詳細

詳細

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 4D17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Nanog. It targets an epitope within 20 amino acids from the N-terminal half. Predicted to react with both isoforms.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of mouse Nanog.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in E14-TG2a cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nanog in E14-TG2a cell lysates.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nanog in Mouse embryonic stem cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nanog in mouse testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nanog in E14-TG2a cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Homeobox protein NANOG (UniProt: Q80Z64; also known as ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog) is encoded by the Nanog (also known as Ecat4, Enk) gene (Gene ID: 71950) in murine species. Nanog is a transcription factor that is involved in the self-renewal of embryonic stem cells (ES) and is a critical factor for the maintenance of the undifferentiated state of pluripotent cells. It binds optimally to the DNA consensus sequence 5′-TAAT[GT][GT]-3′ or 5′-[CG][GA][CG]C[GC]ATTAN[GC]-3′. Its expression is observed in inner cell mass (ICM) of the blastocyst and gonocytes and its expression decreases with ES differentiation. Nanog can autorepress its own expression in differentiating ES cells by binding to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. Overexpression of Nanog in ES cells maintains specific differentiation, however, its overexpression in cancer stem cells is reported to inhibit apoptosis. Together with OCT4, it can activate SNAIL2 and promote metastasis and the EMT phenotype in cancer cells. Nanog undergoes phosphorylation on serine 130 and 131 by casein kinase I and phosphomimetic substitution of serine 130 is shown to abolish its DNA binding and eliminates LIF‐independent self‐renewal. On the other hand, phosphomimetic substitution of serine 131 enhances LIF‐independent self‐renewal but does not affect its DNA binding. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mullin, NP., et al. (2021). FEBS Lett. 595(1); 14-25; Gawlik-Rzemieniewska, N., and Bednarek, I (2016). Cancer Biol. Ther. 17(1); 1-10; Kim, S-H., et al. (2014). Stem Cell Res. 13(1); 1-11).

物理的形状

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

再構成

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法的情報

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable