由来生物
rabbit
品質水準
リコンビナント
expressed in HEK 293 cells
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
1J7, recombinant monoclonal
詳細
recombinant, expressed in HEK 293 cells
製品種目
ZooMAb® learn more
形状
lyophilized
分子量
calculated mol wt 44.42 kDa
observed mol wt ~45 kDa
精製方法
using Protein A
化学種の反応性
human
化学種の反応性（ホモロジーによる予測）
canine, feline, monkey, horse, bovine
包装
antibody small pack of 25 μL
環境により配慮した代替製品の特徴
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
強化検証
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
テクニック
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
アイソタイプ
IgG
エピトープ配列
N-terminal
タンパク質IDアクセッション番号
UniProtアクセッション番号
環境により配慮した代替製品カテゴリ
輸送温度
ambient
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MAP2K2(5605)
詳細
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1J7 is a ZooMAb® Rabbit recombinant monoclonal antibody that detects MEK2. It targets an epitope within 21 amino acids the N-terminal region. Also, displays reactivity with MEK1.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human MEK2.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MEK2 in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from representative lot detected MEK2 in PC12, NIH3T3, and A431 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from representative lot detected MEK2 in HeLa, A431, HepG2, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from representative lot detected MEK2 in human spleen tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MEK2 in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from representative lot detected MEK2 in PC12, NIH3T3, and A431 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from representative lot detected MEK2 in HeLa, A431, HepG2, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from representative lot detected MEK2 in human spleen tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
ターゲットの説明
Dual specificity mitogen-activated protein kinase kinase 2 (UniProt: P36507; also known as EC:2.7.12.2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2) is encoded by the MAP2K2 (also known as MEK2, MKK2, PRKMK2) gene (Gene ID: 5605) in human. MAP kinases are a group of evolutionarily conserved proline-directed protein serine/threonine kinases that are activated in response to a variety of extracellular stimuli and mediate signal transduction from the cell surface to the nucleus. They regulate several physiological and pathological cellular phenomena, including inflammation, apoptotic cell death, oncogenic transformation, tumor cell invasion, and metastasis. They are part of a three-tiered phospho-relay cascade consisting of MAP kinase, a MAP kinase kinase (MEK or MKK) and a MAP kinase kinase kinase (MEKK or MKKK). In this relay system MKKKs phosphorylate and activate MEKs, which in turn phosphorylate and activate MAPKs. MEKs are dual specificity enzymes that phosphorylate hydroxyl side chains of serine/threonine and tyrosine residues in their MAP kinase substrates. MEKs are activated by phosphorylation of serine or threonine residues in their activation loop. MEK2 plays a critical role in mitogen growth factor signal transduction. It phosphorylates a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAPK1/ERK2 and MAPK2/ERK3. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
物理的形状
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
再構成
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法的情報
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ZRB1604-4X25UL:
ZRB1604-25UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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