SRP0170

ACK1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• 別名: Activated Cdc42-associated kinase 1, TNK2, Tyrosine kinase, non-receptor, 2
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected insect cells
• アッセイ: ≥70% (SDS-PAGE)
• 形状: aqueous solution
• 分子量: 68 kDa
• 包装: pkg of 10 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_005781
• UniProtアクセッション番号: Q07912
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... TNK2(10188)

詳細

詳細

ACK1 (activated CDC42 kinase 1) is a non-receptor tyrosine kinase and is present in all human tissues. It belongs to the family of cytoplasmic tyrosine kinases. The protein has a sterile α motif (SAM) domain, a tyrosine kinase domain, a SH3 (src homology 3) domain, a CDC42/RAC-interactive (CRIB) domain, and a region that is homologous to the EGFR (epidermal growth factor receptor) binding domain of Mig6 (mitogen-induced gene 6) and a ubiquitin association domain.
Human Ack1 (GenBank Accession No. NM_005781), full length (amino acids 110-476) with N-terminal GST tag, MW = 68kDa, expressed in Sf9 insect cell systems.

アプリケーション

酵素キネティクスの研究をはじめ、インヒビターのスクリーニングや選択性プロファイリングにもご利用いただけます。

生物化学的／生理学的作用

ACK1 (activated CDC42 kinase 1) was first discovered for its binding to CDC42 (cell division control protein 42 homolog). ACK1 and CDC42 controls cellular attachment and migration. ACK1 is involved in cell survival, proliferation and migration. It is also associated with various cancers. The ACK1 gene is overexpressed in prostate, gastric and pancreatic cancers. It also interacts with the heavy chain of clathrin and might be involved in receptor-mediated endocytosis. ACK1 is a dual specificity kinase towards WASP (Wiskott–Aldrich Syndrome protein). ACK1 is also responsible for the phosphorylation and activation of DBL (an exchange factor for Rho) and Ras/GRF1 (an exchange factor for Ras).

単位の定義

1ユニットは、pH 7.4、30°Cで1分あたり1 pmolのチロシン基質をリン酸化する酵素量と定義されています。

物理的形状

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注した酵素液は希釈せずそのまま-70°Cで保存してください。注:酵素は凍結融解の繰り返しに対して非常に不安定です。