SAB4200353

Anti-acetyl-Histone H4 [Ac-Lys¹²] antibody produced in rabbit

IgG fraction of antiserum

• 別名: Anti-H4, Anti-H4K12ac, Anti-HIST4H4, Anti-Histone cluster 4
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~12 kDa
• 化学種の反応性: mouse, rat, human
• テクニック: immunohistochemistry: 1:100-1:200 using formalin-fixed paraffin-embedded human breast carcinoma; indirect immunofluorescence: 1:250-1:500 using HeLa cells; western blot: 1:500-1:1000 using NIH3T3 cell lysates
• UniProtアクセッション番号: Q16695
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: acetylation (Lys12)
• 遺伝子情報: human ... HIST3H3(8290)

詳細

詳細

Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. It is an important component of chromatin structure.

免疫原

synthetic peptide containing acetylated Lys12 of human histone H4, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H4.

アプリケーション

Anti-acetyl-Histone H4 [Ac-Lys¹²] antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

生物化学的／生理学的作用

Histone H4 can be reversibly acetylated at lysine residues K5, K8, K12 and K16, and the acetylation occurs initially at K16 and then propagates in an N-terminal direction through K12, K8 and K5. Altered acetylation of histone H4 on Lys¹² (H4- K12Ac) is linked to age-dependent memory impairment in mice. Histones are subjected to several covalent modifications, such as phosphorylation, methylation, acetylation and ubiquitination, that affect chromatin structure and regulate chromatin activity. Histone modifications plays an important role in cancer and disease.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable