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由来生物
mouse
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
6B4
monoclonal
形状
liquid
分子量
220 kDa
化学種の反応性
mouse, human
濃度
1.36 mg/mL
テクニック
ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:500-1:3000
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... BRCA1(672)
詳細
The gene BRCA1 is mapped to human chromosome 17q21 and codes for a protein with zinc finger motif and a set of acidic residues. BRCA1 is localized to the nucleus and cytoplasm.
免疫原
BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
アプリケーション
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC, IP, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
生物化学的／生理学的作用
BRCA1-6B4 recognizes full length BRCA1, a 220 kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51. BRCA1 is predominantly expressed in proliferating and differentiating cells and contributes to cellular growth.
特徴および利点
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
物理的形状
Phosphate-buffered saline, no preservative added.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
The nuclear localization sequences of the BRCA1 protein interact with the importin-α subunit of the nuclear transport signal receptor.
Chen C F, et al.
The Journal of Biological Chemistry, 271(51), 32863-32868 (1996)
Location of BRCA1 in human breast and ovarian cancer cells.
Scully R, et al.
Science, 272(5258), 123-123 (1996)
Epidemiology of breast cancer.
Key T J, et al.
Lancet Oncology, 2(3), 133-140 (2001)
Michal Franek et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 64(11), 669-686 (2016-09-30)
DNA damage response (DDR) in ribosomal genes and mechanisms of DNA repair in embryonic stem cells (ESCs) are less explored nuclear events. DDR in ESCs should be unique due to their high proliferation rate, expression of pluripotency factors, and specific
Anuratha Sakthianandeswaren et al.
Cancer discovery, 8(8), 988-1005 (2018-06-09)
ADP-ribosylation is an important posttranslational protein modification that regulates diverse biological processes, controlled by dedicated transferases and hydrolases. Here, we show that frequent deletions (∼30%) of the MACROD2 mono-ADP-ribosylhydrolase locus in human colorectal cancer cause impaired PARP1 transferase activity in
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