形状
buffered aqueous glycerol solution
濃度
10,000 units/mL
輸送温度
wet ice
保管温度
−20°C
特異性
認識配列: 5'-ATTT/AAAT-3'
ライゲーション・再切断アッセイ:2~10倍量のSwa Iで1 μgのλDNA基質を過剰消化した場合、切断率は100%、ライゲーション率は>95%、再切断率は>95%です。
熱失活:65°C、20分間で失活します。
ライゲーション・再切断アッセイ:2~10倍量のSwa Iで1 μgのλDNA基質を過剰消化した場合、切断率は100%、ライゲーション率は>95%、再切断率は>95%です。
熱失活:65°C、20分間で失活します。
その他情報
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
物理的形状
20 mM Tris-HCl溶液(pH 8.0、0.1 mM EDTA、500 mM NaCl、10 mM 2-メルカプトエタノール、50%グリセロール(v/v)含有、4°C)
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Gene, 33(3), 357-361 (1985-01-01)
A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Scandinavian journal of clinical and laboratory investigation, 73(1), 75-81 (2012-12-04)
Left ventricular hypertrophy (LVH) is a common manifestation of cardiovascular disease and has an important prognostic value in patients with end-stage renal disease (ESRD). Vitamin D receptor (VDR) has been intensively investigated, and one of these (BsmI) already has been
Nucleic acids research, 41(5), 3274-3288 (2013-01-29)
Helicobacter pylori is a Gram-negative bacterium that colonizes human stomach and causes gastric inflammation. The species is naturally competent and displays remarkable diversity. The presence of a large number of restriction-modification (R-M) systems in this bacterium creates a barrier against
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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