P7493

抗PCAF抗体 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗p300/CBP結合性因子抗体
• MDL番号: MFCD03791929
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~88 kDa
• 化学種の反応性: canine, human
• テクニック: immunoprecipitation (IP): 5-10 μg using RIPA lysate with ~4x10⁷ human acute T cell leukemia Jurkat cells; microarray: suitable; western blot (chemiluminescent): 1:500 using whole extracts of dog MDCK kidney cells
• UniProtアクセッション番号: Q92831
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... KAT2B(8850)

詳細

詳細

PCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily.

特異性

Anti-PCAF recognizes human and dog PCAF. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.

免疫原

ヒトPCAFのC末端アミノ酸817-832に相当する合成ペプチド(N末端システイン付加)のKLH結合体。

This sequence is identical to mouse PCAF and varies by 2 amino acids residues in chicken.

アプリケーション

Anti-PCAF antibody is also suitable for use microarray, immunoblot, chemiluminescent western blot (1:500 using whole extracts of dog MDCK kidney cells), and immunoprecipitation (5-10 μg using RIPA lysate with approx. 4x10,000,000 human acute T cell leukemia Jurkat cells).

Anti-PCAF antibody produced in rabbit has been used in western blot and immunoprecipitation.

生物化学的／生理学的作用

PACF (p300/CBP association factor) functions as a transcriptional coactivator in association with p300 and CBP (CREB-binding protein). PCAF share high degree of sequence similarity with human and mouse GCN5 HAT (Histone acetyltransferases). PACF mediates the acetylation of core histone. It is also known for the acetylation of non-histone proteins such as p53, MyoD, TFIIE-b, TFIIE, GATA-1, EKLF, HMGI (Y), and several other cellular and viral proteins. PCAF possesses chromatin modification activity and modulation of transcription factors for the activation of transcription. It also promotes cell growth and development as myogenesis and also contribute to receptor-mediated cell signaling or activation.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable