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Polythymidylicacid sodium salt has been used as a control to compare with poly(A) in aforce-extension experiment to investigate differences in mechanical properties. Polythymidylic acid (Poly(dT)) may be used to study the characteristics of single-stranded DNA binding proteins.
生物化学的／生理学的作用
Polythymidylicacid, also known as poly-deoxythymidine (poly-dT), has various applications inmolecular biology research. One of its most common uses is for mRNApurification, where it is utilized to isolate mRNA from a mixture of RNAmolecules for the preparation of cDNA libraries. In addition, poly-dT can beused as a tool for the detection of microbial contamination in water samples.It can also be used for the purification of mRNA-protein fusion molecules inmRNA Display selections.
単位の定義
1 unitは、水1.0 mL中に1.0の A260 を生じる酵素量です(1 cm光路)。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
P6905-25UN:
P6905-5UN:
P6905-5UN-PW:
P6905-BULK:
P6905PROC:
P6905-50UN:
P6905-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Gregor Witte et al.
Nucleic acids research, 33(5), 1662-1670 (2005-03-23)
The highly conserved bacterial single-stranded DNA-binding (SSB) proteins play an important role in DNA replication, repair and recombination and are essential for the survival of the cell. They are functional as tetramers, in which four OB(oligonucleotide/oligosaccharide binding)-folds act as DNA-binding
Józef Kur et al.
Acta biochimica Polonica, 52(3), 569-574 (2005-08-06)
Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea and eukarya. The SSBs share a common core ssDNA-binding domain with a conserved OB (oligonucleotide/oligosaccharide binding) fold. This ssDNA-binding domain was presumably present in
Paweł Filipkowski et al.
Extremophiles : life under extreme conditions, 10(6), 607-614 (2006-08-10)
We report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus radiopugnans (DrpSSB). PCR-derived DNA fragment containing the complete structural gene for DrpSSB protein was cloned and expressed in Escherichia coli. The
Byul Nim Oh et al.
Dalton transactions (Cambridge, England : 2003), 40(24), 6494-6499 (2011-05-13)
In this study, an assay to quantify the presence of mercuric ions and methyl mercury by double-stranded DNA containing a poly(dT) sequence was developed using a light switch compound, Ru(phen)(2)(dppz)(2+) (1), which is known to intercalate into double-stranded DNA. Upon
Alexander G Kozlov et al.
Biochemistry, 49(38), 8266-8275 (2010-08-28)
Deinococcus radiodurans single-stranded (ss) DNA binding protein (DrSSB) originates from a radiation-resistant bacterium and participates in DNA recombination, replication, and repair. Although it functions as a homodimer, it contains four DNA binding domains (OB-folds) and thus is structurally similar to
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