OGS3157
PSF-OXB20-NH2-PELB - PELB SECRETION PLASMID
plasmid vector for molecular cloning
別名:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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この商品について
NACRES:
NA.85
UNSPSC Code:
12352200
Promoter:
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
Promoter activity: constitutive
Promoter type: bacterial
Origin of replication:
pUC (500 copies)
Bacteria selection:
kanamycin
Reporter gene:
none
Peptide cleavage:
no cleavage
recombinant
expressed in E. coli
form
buffered aqueous solution
mol wt
size 3914 bp
bacteria selection
kanamycin
origin of replication
pUC (500 copies)
peptide cleavage
no cleavage
peptide tag location
N-terminal
promoter
Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial
reporter gene
none
secretion signal
PelB
shipped in
ambient
storage temp.
−20°C
General description
This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.
About the Peptide Tag:This plasmid contains an Pectate Lyase B (PelB) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
About the Peptide Tag:This plasmid contains an Pectate Lyase B (PelB) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.
Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Other Notes
To view sequence information for this product, please visit the product page
保管分類
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
OGS3157-5UG:
jan