MP0025

Venor^™GeM マイコプラズマ検出キット、PCR

• 別名: PCR mycoplasma detection kit
• UNSPSCコード: 12352207
• NACRES: NA.75

特性

• 使用法: kit sufficient for 25 tests
• 包装: pkg of 1 kit
• 保管条件: dry at room temperature
• テクニック: PCR: suitable
• アプリケーション: detection; microbiology
• 保管温度: 2-8°C

詳細

詳細

The Venor^™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

アプリケーション

The PCR-based Venor^™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.

構成

Taq DNAポリメラ-ゼは付属していません。D9307, Taq DNAポリメラ-ゼと組み合わせての使用に最適化されています。

保管および安定性

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

法的情報

Venor is a trademark of Minerva Biolabs GmbH

キットの構成要素のみ

• Positive Control
• Negative Control
• PCR 10X Reaction Buffer 500 mL/vial
• Primer/Nucleotide Mix 1 mL/vial

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3