M7802

モノクロナール抗MAPキナーゼ, 活性化/モノリン酸化 (リン酸化トレオニン ERK-1&2) マウス宿主抗体

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD01633612
• Conjugate: unconjugated
• Clone: ERK-PT115, monoclonal
• Application: ARR, ELISA (c), ICC, WB
• Citations: 16

特性

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ERK-PT115, monoclonal
• form: buffered aqueous solution
• mol wt: antigen, ERK-1 44 kDa, antigen, ERK-2 42 kDa
• species reactivity: rat, human
• concentration: ~2 mg/mL
• technique(s): capture ELISA: suitable, immunocytochemistry: suitable, microarray: suitable, western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol
• isotype: IgG1
• UniProt accession no.: P28482
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAPK1(5594), MAPK3(5595) ; rat ... Mapk1(116590), Mapk3(50689)

詳細

General description

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

Immunogen

リン酸化型のERK活性化ル-プに相当する合成ペプチド(HTGFLpTEYVAT)。

Application

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:

• immunoblotting
• western blotting
• immunofluorescence
• enzyme linked immunosorbent assay (ELISA)
• immunochemistry

Biochem/physiol Actions

抗体は、スレオニン一リン酸化型および活性化された二リン酸化型のMAPキナ-ゼ(ERK-1, ERK-2)と特異的に反応します。非リン酸化型のMAPキナ-ゼ分子や二リン酸化型のJNKおよびp38 MAPKは認識されません。抗体が認識するエピト-プには、活性化MAPキナ-ゼの調節部位のリン酸化スレオニン残基(ERK-2の場合Thr¹⁸³)が含まれています。

Physical form

0.01M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable