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由来生物
rabbit
品質水準
結合体
unconjugated
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous solution
分子量
antigen ~54 kDa
化学種の反応性
human
テクニック
western blot: 1:250-1:500
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MLKL(197259)
関連するカテゴリー
詳細
MLKL (mixed lineage kinase domain-like) is a protein that belongs to the protein kinase superfamily. It facilitates necrosis signaling downstream of the kinase RIP3 (receptor-interacting serine-threonine kinase 3) . Anti-MLKL (58-70) antibody can be used in western blotting. Rabbit anti- MLKL (58-70) antibody reacts specifically with MLKL protein.
MLKL is encoded by the gene mapped to human chromosome 16q23. Activated MLKL is localized on the cell membrane.
免疫原
synthetic peptide corresponding to amino acids 58-70 of human MLKL.
アプリケーション
Anti-MLKL (58-70) antibody produced in rabbit has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunocytochemistry (1 paper)
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
western blot: 1:250-1:500
生物化学的／生理学的作用
Mixed lineage kinase domain-like protein (MLKL) primarily causes receptor-interacting protein (RIP) kinase-dependent necroptosis. However, during hepatitis, it results in programmed hepatocellular necrosis, which is independent of RIPK3. MLKL also participates in endosomal trafficking and in the formation of extracellular vesicles.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
M6697-BULK:
M6697-200UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly.
Selmi T, et al.
BMC Cancer, 15(1), 357-357 (2015)
Characterization of RIPK3-mediated phosphorylation of the activation loop of MLKL during necroptosis.
Rodriguez DA, et al.
Cell Death and Differentiation, 23(1), 76-76 (2016)
MLKL activation triggers NLRP3-mediated processing and release of IL-β independently of gasdermin-D
Gutierrez KD, et al.
Journal of Immunology, 47(1), 1601757-1601757 (2017)
Sara R Oliveira et al.
Cell death discovery, 4, 10-10 (2018-08-01)
Necroptosis is a regulated form of necrosis, which may be critical in the pathogenesis of neurodegenerative diseases. Neuroinflammation, characterized by the activation of glial cells such as microglia, is closely linked with neurodegenerative pathways and constitutes a major mechanism of
Giovanni Quarato et al.
Molecular cell, 61(4), 589-601 (2016-02-09)
Necroptosis is a cell death pathway regulated by the receptor interacting protein kinase 3 (RIPK3) and the mixed lineage kinase domain-like (MLKL) pseudokinase. How MLKL executes plasma membrane rupture upon phosphorylation by RIPK3 remains controversial. Here, we characterize the hierarchical
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