M5698
Anti-MIB2 (C-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
別名:
Anti-Mindbomb homolog 2, Anti-Novel zinc finger protein, Anti-Novelzin, Anti-Skeletrophin, Anti-Zinc finger ZZ type with ankyrin repeat domain protein 1
ログイン組織・契約価格を表示する
すべての画像(1)
About This Item
UNSPSCコード:
12352203
NACRES:
NA.41
由来生物
rabbit
結合体
unconjugated
抗体製品の状態
IgG fraction of antiserum
抗体製品タイプ
primary antibodies
クローン
polyclonal
形状
buffered aqueous solution
分子量
antigen ~110 kDa
化学種の反応性
human, rat
テクニック
western blot: 1:500-1:1,000 using lysates of rat skeletal muscle
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MIB2(142678)
詳細
Mindbomb homolog 2 (MIB2) which is also known as skeletrophin, is an ubiquitin ligase. The gene encoding it is localized on human chromosome 1p36.32.
Mouse MIB1 and MIB2 share 52% similarity in their amino acid sequences and contain a similar domain organization.These include two homologous to the E6-AP carboxyl terminus (HECT) domains. It also comprises RCC1-like domain (RLD) domain containing E3 ubiquitin protein ligase 2 (herc2)/mib domains.Other domains associated with MIB2 are one ZZ zinc finger, one mib repeat, ankyrin repeats, and two interesting new gene (RING) domains. MIB2 is highly expressed in adult tissues.
特異性
Anti-MIB2 (C-terminal) specifically recognizes human and rat MIB2.
免疫原
synthetic peptide corresponding to amino acides 950-966 of human MIB2 conjugated to KLH. The corresponding sequence differs by one amino acid in mouse.
アプリケーション
Anti-MIB2 (C-terminal) antibody produced in rabbit may be used in immunoblotting.
生物化学的／生理学的作用
Disruption of the MIB2 gene results in exencephaly and appears to be less essential in mouse development.
Mindbomb homolog 2 (MIB2) takes part in the ubiquitination of Notch ligands like Jagged-2 (JAG2). It binds to actin and CYLD (cylindromatosis (turban tumor syndrome)), which is an ubiquitin hydrolase. It has also been shown that MIB2 negatively regulates the transcription of Met oncogene.
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
保管および安定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 製品選択ツール.
保管分類コード
10 - Combustible liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
M5698-25UL:
M5698-VAR:
M5698-BULK:
M5698-200UL:
IXO14728:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Katrin Domsch et al.
PloS one, 12(3), e0173733-e0173733 (2017-03-11)
The proper differentiation and maintenance of myofibers is fundamental to a functional musculature. Disruption of numerous mostly structural factors leads to perturbations of these processes. Among the limited number of known regulatory factors for these processes is Mind bomb2 (Mib2)
Bon-Kyoung Koo et al.
PloS one, 2(11), e1221-e1221 (2007-11-29)
The Notch signaling pathway is an evolutionarily conserved intercellular signaling module essential for cell fate specification that requires endocytosis of Notch ligands. Structurally distinct E3 ubiquitin ligases, Neuralized (Neur) and Mind bomb (Mib), cooperatively regulate the endocytosis of Notch ligands
Jiang I Wu et al.
Genesis (New York, N.Y. : 2000), 45(11), 722-727 (2007-11-08)
Mib1 and Mib2 ubiquitin ligases are very similar in their domain construction. They partake in the Notch signaling pathway by ubiquitinating the Notch receptors Delta and Jagged prior to endocytosis. We have created a targeted mutation of Mib2 and show
T Takeuchi et al.
Oncogene, 25(53), 7059-7069 (2006-05-23)
Skeletrophin (mindbomb homolog 2 (MIB2)) is a RING (Really Interesting New Gene) finger-dependent ubiquitin ligase, which targets the intracellular region of Notch ligands. A previous immunohistochemical study demonstrated that skeletrophin was downregulated in many melanomas. In the present study, we
Tamotsu Takeuchi et al.
DNA and cell biology, 24(5), 339-344 (2005-05-05)
Recent experiments have revealed that aberrant functionality of the chromatin remodeling complex is related to tumorigenicity in various malignant tumors. Skeletrophin is an actin-binding cytoskeleton-related molecule, which is induced by the overexpression of truncated human SWI1 (SMARCF1). Human SWI1 is
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）