由来生物
rabbit
品質水準
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
フォーム
buffered aqueous glycerol solution
分子量
antigen 48 kDa
交差性
rat, mouse, human
テクニック
flow cytometry: 1:200
microarray: suitable
western blot (chemiluminescent): 1:1,000 using extract of NIH3T3 anisomycin or UV-treated cells
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
phosphorylation (pSer63)
遺伝子情報
human ... JUN(3725)
mouse ... Jun(16476)
特異性
The antibody does not react with the corresponding phosphorylated form of JunD or JunB.
免疫原
synthetic phosphoserine63 peptide corresponding to residues around Ser63 of human c-Jun, conjugated to KLH.
アプリケーション
Anti-phospho-c-Jun (pSer63) antibody produced in rabbit has been used in microarray analysis to identify the changes in the level of cellular proteins in cells inoculated with Equine influenza virus.
生物化学的／生理学的作用
The protooncogene c-Jun encodes a member of the jun family that forms a component of the transcription factor AP-1 (Activator Protein-1). It dimerizes with a member of the Fos family via the leucine-zipper motif to form AP-1, a factor involved in binding to TPA (12-O-tetradecanoylphorbol 13-acetate) response element (TRE) of various genes that include human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. The dimers formed are involved in the activation of AP-1 dependent genes. AP-1 regulates the transcription of mammalian DDI (DNA-damage-inducible) genes.
物理的形状
Solution in 10 mM HEPES sodium, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin, and 50% glycerol.
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保管分類コード
10 - Combustible liquids
K Ryder et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(5), 1500-1503 (1989-03-01)
The protooncogene c-jun encodes a component of the transcription factor AP-1. Both murine c-jun and a related gene (jun-B) are rapidly activated in BALB/c3T3 cells by serum growth factors. We report here the cloning and analysis of a cDNA encoding
D A Brenner et al.
Nature, 337(6208), 661-663 (1989-02-16)
Tumour necrosis factor-alpha (TNF-alpha) is secreted by macrophages in response to inflammation, infection and cancer. Sublethal doses of recombinant TNF-alpha to rats causes cachexia, anaemia and inflammation. TNF-alpha plays a major part in tissue inflammation and remodelling by stimulating production
Y Devary et al.
Molecular and cellular biology, 11(5), 2804-2811 (1991-05-01)
Exposure of mammalian cells to DNA-damaging agents leads to activation of a genetic response known as the UV response. Because several previously identified UV-inducible genes contain AP-1 binding sites within their promoters, we investigated the induction of AP-1 activity by
P Angel et al.
Cell, 55(5), 875-885 (1988-12-02)
Binding of the human transcription factor Jun/AP-1 to a conserved 8 bp nucleotide sequence (TRE) is responsible for increased transcription of different cellular genes in response to tumor promoters, such as TPA, and serum factors. Enhanced Jun/AP-1 activity in TPA-stimulated
The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation.
P Angel et al.
Biochimica et biophysica acta, 1072(2-3), 129-157 (1991-12-10)
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