フォーム
liquid
包装
pkg of 220 mL
メーカー／製品名
Cytiva RPN510
輸送温度
dry ice
保管温度
2-8°C
詳細
Amerlex-M Donkey Anti-Rabbit Reagent.
アプリケーション
Amerlex M system is a magnetic particle separation system. system for use in immunoassay. It combines the advantages of second antibody solid phase separation with liquid phase kinetics. Its use eliminates centrifugation during the separation of free and bound ligand. Amerlex-M donkey anti-rabbit reagent consists of magnetizable polymer beads coated with second antibody. The use of this reagent enables phase separation by introduction of a magnetic field. It is used where the primary antibody is of rabbit origin and is particularly suitable for iodine-125 radioimmunoassays as bound counts end up in the pellet. An Amerlex-M Separator, consisting of detachable 50-tube rack and magnetic base, is available for use with Amerlex-M assays. Following addition of the donkey anti-rabbit reagent to the assay tubes, the rack containing the tubes is clipped onto the magnetic base.
Primary antibody - with and without labelled ligand - bound to the second antibody on the beads rapidly migrates to the base of the tube, enabling separation of phases by decantation. Measurement of radioactivity in the pellet enables the amount of labelled analyte in the bound fraction to be calculated. The concentration of unlabelled analyte is determined by interpolation from a standard curve. 500 μL of Amerlex-M donkey anti-rabbit reagent is sufficient to bind at least 5 μg rabbit IgG. The reagent is supplied in a phosphate buffered solution, pH 7.4. Although the Amerlex-M reagent is designed for use in a magnetic separation protocol, conventional centrifugation can be used if preferred. Instruction on the use of centrifugation is given in the separation protocol. Note: Given the variation in assay conditions and protocols, it remains the responsibility of the user to optimize the use of Amerlex-M reagent in a particular assay.
Primary antibody - with and without labelled ligand - bound to the second antibody on the beads rapidly migrates to the base of the tube, enabling separation of phases by decantation. Measurement of radioactivity in the pellet enables the amount of labelled analyte in the bound fraction to be calculated. The concentration of unlabelled analyte is determined by interpolation from a standard curve. 500 μL of Amerlex-M donkey anti-rabbit reagent is sufficient to bind at least 5 μg rabbit IgG. The reagent is supplied in a phosphate buffered solution, pH 7.4. Although the Amerlex-M reagent is designed for use in a magnetic separation protocol, conventional centrifugation can be used if preferred. Instruction on the use of centrifugation is given in the separation protocol. Note: Given the variation in assay conditions and protocols, it remains the responsibility of the user to optimize the use of Amerlex-M reagent in a particular assay.
特徴および利点
- Magnetizable polymer beads coated with secondary antibody.
- Simple magnetic separation eliminates centrifugation.
- Minimizes hands-on time by reducing tube handling.
- Sufficient for 400 tubes in a typical assay.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
保管分類コード
12 - Non Combustible Liquids
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