G115030

DMT-dG(dmf) ホスホロアミダイト

configured for ABI

• 別名: DMT-dG（dmf）アミダイト, N-[（ジメチルアミノ）メチレン]-5’-O-[ビス（4-メトキシフェニル）フェニルメチル]-2’-デオキシグアノシン, 3’-[2-シアノメチルN,N-ビス（1-メチルエチル）ホスホルアミダイト], N2-ジメチルホルムアミジン-5’-O-（4,4’-ジメトキシトリチル）-2’-デオキシグアノシン-3’-O-[O-（2-シアノエチル）-N,N’-ジイソプロピルホスホルアミダイト]
• 実験式（ヒル表記法）: C₄₃H₅₃N₈O₇P
• CAS番号: 330628-04-1
• 分子量: 824.90
• MDL番号: MFCD00797420
• UNSPSCコード: 12352200
• PubChem Substance ID: 329799822
• NACRES: NA.51

特性

• タイプ: for DNA synthesis
• 製品種目: Proligo Reagents
• アッセイ: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• 形状: powder
• テクニック: oligo synthesis: suitable
• 色: white to off-white
• λ: conforms (UV/VIS Identity)
• 適合性: configured for ABI
• 核酸プロファイル: base: deoxyguanosine; base protecting group: DMF; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• 保管温度: -10 to -25°C
• SMILES記法: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(\N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
• InChI: 1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1
• InChI Key: YRQAXTCBMPFGAN-UJASEYITSA-N

詳細

詳細

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamidine (dmf) base protecting group enables rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.

Key Features of dG(dmf)

• dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for ABI Synthesizers.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable