由来生物
rabbit
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse, rat
濃度
1.0-3.0 mg/mL
テクニック
direct immunofluorescence: 2-5 μg/mL using human HEK-293T or HeLa cells
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... FLOT1(10211)
mouse ... Flot1(14251)
rat ... Flot1(64665)
詳細
Flotillins are lipid raft-associated proteins belonging to the integral membrane proteins. It is associated in different biological processes such as endocytosis, phagocytosis, neuronal regeneration, insulin signaling etc. It exhibits an evolutionarily conserved domain called the prohibitin homology (PHB) domain or SPFH domain (stomatin/prohibitin/flotillin/HflK/C domain). The lipid recognition motif present in the PHB domain, is responsible for targeting proteins to the plasma membrane. Flotillin protein mainly possess two forms, flotillin 1 and 2. They are also known as reggie-2 and 1, respectively.
免疫原
synthetic peptide corresponding to amino acid residues 413-428 of mouse flotillin 1, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.
アプリケーション
Anti-Flotillin 1-CY3™ antibody is suitable for direct immunofluorescence at a concentration of 2-5μg/mL using human HEK-293T or HeLa cells
生物化学的／生理学的作用
Flotillin 1 is directly connected to the plasma membrane. The association of flotillin 1 depends on the palmitoylation in a conserved Cys34 residue, whereas the association of flotillin 2 depends on both myristolation and palmitoylation. Both the form possess a complementary tissue distribution. Flotillin 1 is mainly expressed in striated muscle tissues, adipose tissue and lung, whereas flotillin 2 is much widely found but absent from skeletal muscle. It has been reported that flotillin 1 act as a molecular link between lipid rafts of the plasma membrane and a multimeric signaling complex at the actin cytoskeleton.
物理的形状
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
法的情報
Cy3 is a trademark of Cytiva
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
F8931-BULK:
F8931-25UL:
F8931-200UL:
F8931-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Jun Liu et al.
The Journal of biological chemistry, 280(16), 16125-16134 (2005-02-17)
Flotillin-1 is a lipid raft-associated protein that has been implicated in various cellular processes. We examined the subcellular distribution of flotillin-1 in different cell types and found that localization is cell type-specific. Flotillin-1 relocates from a cytoplasmic compartment to the
D Volonte et al.
The Journal of biological chemistry, 274(18), 12702-12709 (1999-04-23)
Caveolae are vesicular organelles that represent a subcompartment of the plasma membrane. Caveolins and flotillins are two families of mammalian caveolae-associated integral membrane proteins. However, it remains unknown whether flotillins interact with caveolin proteins to form a stable caveolar complex
Isabel C Morrow et al.
The Journal of biological chemistry, 277(50), 48834-48841 (2002-10-09)
Flotillins are lipid raft-associated proteins, which have been implicated in neuronal regeneration and insulin signaling. We now show that newly synthesized flotillin-1 reaches the plasma membrane via a Sar1-independent and brefeldin A-resistant targeting pathway. Consistent with post-translational membrane association of
P E Bickel et al.
The Journal of biological chemistry, 272(21), 13793-13802 (1997-05-23)
Caveolae are plasmalemmal microdomains that are involved in vesicular trafficking and signal transduction. We have sought to identify novel integral membrane proteins of caveolae. Here we describe the identification and molecular cloning of flotillin. By several independent methods, flotillin behaves
Carolin Neumann-Giesen et al.
The Biochemical journal, 378(Pt 2), 509-518 (2003-11-06)
The reggie protein family consists of two proteins, reggie-1 and -2, also called flotillins, which are highly ubiquitous and evolutionarily conserved. Both reggies have been shown to be associated with membrane rafts and are involved in various cellular processes such
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