F7182

線維芽細胞活性化タンパク質α ヒト

recombinant, expressed in Sf21 cells, ≥95% (SDS-PAGE)

• 別名: FAP, セプラーゼ
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥1800 units/μg protein
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in Sf21 cells

特性

• recombinant: expressed in Sf21 cells
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• form: solution
• specific activity: ≥1800 units/μg protein
• impurities: ≤1.0 EU/μg endotoxin
• shipped in: dry ice
• storage temp.: −70°C

詳細

General description

Fibroblast activation protein alpha (FAPa) is a type II transmembrane serine protease. It is present as a homodimeric integral protein with dipeptidyl peptidase IV like fold. It has an α/β-hydrolase domain and an eight-bladed β-propeller domain.[1] It is not expressed in normal tissue. It is only expressed by activated fibroblast in response to pathologic situations. It is expressed in several pathogenic sites including cancer, fibrosis, arthritis, wounding, or inflammation.[2]

Application

FAPa may be used to study the role in coordinating the stromal response to cancer, in angiogenesis, and modulation of the antitumor immune response.[2]

Biochem/physiol Actions

FAPはII型膜貫通型セリンプロテアーゼであり、プロリルペプチド結合(Pro-Xaa)に対する基質特異性を有しています。本エンドペプチダーゼはゼラチンを切断し、組織リモデリング、腫瘍浸潤や創傷治癒において役割を果たすと考えられています。

FAPα has in vitro dipeptidyl peptidase activity and collagenolytic activity. It cleaves N-terminal dipeptides from polypeptides and can degrade gelatin and type I collagen. It has also been reported that FAPa has a tumor suppressor activity.[1]

Physical form

25 mM Tris、0.25 M NaCl(pH 8.0)で調製した溶液。

Other Notes

1 unitは、pH 7.5、25°C、1分間に、1 pmolのベンジルオキシカルボニル-Gly-Pro-7-アミド-4-メチルクマリン(ZP-AMC)を、Z-gly-Proと7-アミノ-4-メチルクマリン(AMC)に変換する酵素量です。

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable