F2677

フリン ヒト

≥2,000 unit/mL, buffered aqueous solution, recombinant, expressed in baculovirus infected Sf9 cells

• 別名: Dibasic-processing enzyme, Furin convertase, PACE, Paired basic amino acid residue-cleaving enzyme
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD03456906
• Recombinant: expressed in baculovirus infected Sf9 cells
• Concentration: ≥2,000 unit/mL

特性

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Segment: 200
• form: buffered aqueous solution
• mol wt: 57 kDa
• concentration: ≥2,000 unit/mL
• UniProt accession no.: P09958
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... FURIN(5045)

詳細

Application

Furin is capable of cleaving precursors of a wide variety of proteins, including growth factors, serum proteins, including proteases of the blood-clotting and complement systems, matrix metalloproteinases, receptors, viral-envelope glycoproteins, and bacterial exotoxins, typically at sites marked by the consensus sequence Arg-Xaa-(Lys/Arg)-Arg.[1]

Biochem/physiol Actions

フリンはゴルジ体に局在する二塩基性エンドプロテア-ゼです。哺乳動物細胞の分泌やエンドサイト-シスの経路において多くの前駆体タンパク質がタンパク質分解により成熟する過程に関与しています。

Furin is a dibasic endoprotease that is localized in the Golgi apparatus. It has a molecular mass of 52.7 kDa. It is responsible for the proteolytic maturation of many precursor proteins in the secretory and endocytic pathways of mammalian cells.[2] Furin cleaves precursor proteins at their paired basic amino acid processing sites. Some substrates of furin include von Willebrand factor, transforming growth factor beta 1 precursor, pro-beta-secretase and proparathyroid hormone.

Physical form

10 mM MES溶液(25°CでpH 7.0、1 mM CaCl₂、50% グリセロールを含有)。

Preparation Note

切断型ヒトフリンを発現する組換え体バキュロウイルスを感染させたSpodoptera frugiperda, Sf9細胞から単離しました。

Other Notes

One unit is defined as the amount of enzyme required to cleave 25 μg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 μl.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable