This serum product is collected from USDA approved abbatoirs and pooled for production. Analytical specifications for Endotoxin, Total Protein, Osmolality, and pH are assigned, and these values reflect a high level of consistency. The maximum allowable hemoglobin content is 25 mg/dL. This value, as well as other measured components, may vary significantly from lot to lot. Note that samples of available lots may be requested for screening purposes. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/f0926#product-documentation
F0926
ウシ胎児血清
USDA approved, sterile-filtered, suitable for cell culture
Fetal Bovine Serum
別名:
FBS, FCS, 血清
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|
この商品について
Biological source:
bovine fetus
Sterility:
sterile-filtered
Shipped in:
dry ice
現在、価格および在庫状況を閲覧できません。
biological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
quality
USDA approved
composition
Hemoglobin, ≤25 mg/dL
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
ウシ胎児血清（FBS）またはFCSは、血液の自然凝固後に残った血液分画です。畜殺場における閉鎖系での採血によって、ウシ胎児から得られます。in vitroでの真核細胞の培養に最も広く使用される血清サプリメントです。
Application
USDA(米国農務省)承認製品(Approved Sourcing)は、牛肉製品の米国向け輸出をUSDAにより承認された、米国外の諸国で収集された血液から製造されたFBSを指します。米国向け輸出の適格国には、オーストラリア、カナダ、チリ、コスタリカ、ホンジュラス、アイスランド、日本、メキシコ、ニュージーランド、ニカラグア、ウルグアイが挙げられます。米国外諸国由来の血清は、発売前に外来ウイルスが存在しないことをUSDAが検査します。
USDAの承認した非米国産FBSは、幅広い細胞培養アプリケーションに使用されます。FBSは細胞培養において、定義されていない多くの増殖促進および生存強化因子を提供します。
ウシ胎児血清は以下の細胞培養に使用されています：
ウシ胎児血清は以下の細胞培養に使用されています：
Analysis Note
- エンドトキシンおよびヘモグロビン試験済み
- 細菌、ウイルス、マイコプラズマの存在の有無については検査済みです
- 無菌条件下にて0.1マイクロン膜で3回ろ過済みです
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当該品目 | |||
|---|---|---|---|
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| composition Hemoglobin, ≤25 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition hemoglobin, ≤20 mg/dL | composition Hemoglobin, ≤25 mg/dL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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保管分類
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the lot-to-lot consistency of this product?
1 回答-
役に立ちましたか？
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Is this item heat inactivated?
1 回答-
This product is not heat inactivated.
Please view product F4135 for a heat-inactivated Fetal Bovine Serum:
https://www.sigmaaldrich.com/product/sigma/f4135.役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
1 回答-
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
役に立ちましたか？
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Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?
1 回答-
FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.
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