F0772

モノクローナル抗CD8抗体-FITC標識 マウス宿主抗体

clone UCHT-4, purified immunoglobulin, buffered aqueous solution

• 別名: モノクローナル抗CD8抗体
• NACRES: NA.44
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: UCHT-4, monoclonal
• Application: FACS
• Citations: 7

特性

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UCHT-4, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: 10 μL using 1 × 10⁶ cells
• isotype: IgG2a
• UniProt accession no.: P10966
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD8A(925), CD8B(926)

詳細

General description

CD8は、2本の鎖 (α/α、 α/β) からなる複合体で、Class I MHC分子のレセプタ-です。このレセプタ-の細胞質部位は、周辺のタンパク質をリン酸化するp56^lckタンパク質チロシンキナ-ゼと会合しています。CD8は、成熟T細胞(細胞傷害性/サプレッサ-T細胞)の約3分の1上に強く発現しています。

Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with human thymocytes followed by peripheral blood T cells. The CD8 antigen is strongly expressed on approximately one-third of mature T cells (cytotoxic/suppressor T cells). Monoclonal Anti-CD8 does not stain B lymphocytes, monocytes, or granulocytes.

Immunogen

ヒト胸腺細胞、続いて末梢血 T細胞

Application

FITC Monoclonal Anti-Human CD8 may be used for:

• Immunofluorescent staining,
• Enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids,
• Identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues,
• Analysis of cell mediated cytotoxicity,
• Studies of immunoregulation in health and disease,
• Investigation of NK cells, Complement mediated cytolysis of CD8 expressing cells

Biochem/physiol Actions

ヒトT細胞傷害性/サプレッサ-T細胞表面の糖タンパク質であるCD8を認識します。懸濁状態で胸腺細胞の約90%が染色されます。胸腺切片において、皮質および髄質の胸腺細胞の双方が染色されます。NK細胞サブセットは、CD8を弱く発現します。本抗体が認識するエピト-プは、日常的なホルマリン固定およびパラフィン包埋により損傷されやすいものです。
第3回ワ-クショップ:コ-ド番号567567

CD8a (Cluster of Differentiation 8a) is a human gene. CD8 bind to class I MHC and the co-receptors during the process of cytotoxic T-cell activation. T cells that express T cell receptor (TCR) complexes, which lack CD3 δ, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 δ couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8⁺ T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).

Physical form

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

Preparation Note

フルオレセインイソチオシアネ-トアイソマ-I(FITC)とのコンジュゲ-ションにより作製しています。この緑色色素は励起波長495, 蛍光波長525 nmでの測定が効果的です。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• 一体何が起きているの？: nwg
• 引火点 (F): Not applicable
• 引火点（°C）: Not applicable