E8022
pRc/RSV Expression vector
set of expression vectors powered by either the CMV2 or the RSV promoter
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
20 μg | カートの在庫状況を確認する | ￥115,000 |
この商品について
￥115,000
カートの在庫状況を確認する
高品質はそのまま、さらにお手頃な価格になりました
grade
Molecular Biology
form
buffered aqueous solution
storage temp.
−20°C
General description
pRc and pRSV Supercoiled Expression Vectors (5.2 kb) are for protein expression in mammalian cells. Each vector contains:
The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.
The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.
The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.
- The polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
- The SV40 replication origin for episomal replication
- The f1 origin for rescue of the sense strand
- The neomycin resistance gene, and
- An ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.
The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.
The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.
Biochem/physiol Actions
The MCS contains unique ApaI (CMV2 only), HindIII, NotI, SpeI (RSV only), and XbaI restriction sites, plus redundant BstXI sites.
Features and Benefits
Supercoiled vectors for protein expression in mammalian cells. Both of these vectors contain:
- the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
- the SV40 replication origin for episomal replication
- the f1 origin for rescue of the sense strand
- the neomycin resistance gene, and
- an ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
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当該品目 | ||
|---|---|---|
| form buffered aqueous solution | form - | form - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| grade Molecular Biology | grade - | grade Molecular Biology |
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
E8022-20UG: + E8022-BULK: + E8022-VAR:
jan