E6154

抗リン酸化-上皮成長因子受容体 (pTyr¹⁰⁶⁸) ウサギ宿主抗体

affinity isolated antibody, buffered aqueous glycerol solution

• 別名: 抗EGFR, リン酸化型(pTyr¹⁰⁶⁸)抗体
• UNSPSC Code: 51111800
• NACRES: NA.41
• MDL number: MFCD03845325
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 7

特性

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen 170 kDa
• species reactivity: human, rat
• technique(s): western blot: suitable
• UniProt accession no.: P00533
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr1068)
• Gene Information: human ... EGFR(1956)

詳細

General description

The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R is present on many cell types of epithelial and mesenchymal lineages.

The gene EGFR (Epidermal Growth Factor Receptor) is mapped to human chromosome 7p11.2.

Immunogen

EGFレセプタ-のチロシン1068を含む領域由来の合成リン酸化ペプチド。

Application

Anti-phospho-EGFR [pTyr¹⁰⁶⁸] antibody may be used at a working dilution of 1:1000 for immunoblotting using NIH3T3 cells expressing human EGF R, stimulated with EGF. The antibody has been in detection of EGF R [pTyr¹⁰⁶⁸] in human lymphocytes.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is capable of binding transforming growth factor-a and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Phosphorylation of Tyr1068 occurs by autophosphorylation of EGF R and is required for the binding of Grb2 and activation of ERK1/2 pathway Anti-phospho-EGFR [pTyr1068] specifically recognizes human and rat EGFR phosphorylated at tyrosine 1068.

Physical form

D-PBS (pH 7.4, 50%グリセロ-ル, 1mg/mL BSA(IgG/プロテア-ゼ不含), 0.05%アジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)