E0412

カルボキシエステラーゼ2 ヒト

recombinant, expressed in baculovirus infected BTI insect cells

• 別名: CES2, CES2A1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.1.1.1 (BRENDA, IUBMB)
• Specific activity: ≥500 units/mg protein
• Recombinant: expressed in baculovirus infected BTI insect cells

特性

• recombinant: expressed in baculovirus infected BTI insect cells
• Quality Segment: 200
• form: liquid
• specific activity: ≥500 units/mg protein
• mol wt: 60 kDa
• weight: (0.5 ml)
• shipped in: dry ice
• storage temp.: −70°C

詳細

General description

Carboxylesterase 2 human gene is mapped to human chromosome 16q22.1.[1]

Carboxylesterase belongs to the α/β hydrolase fold family and is involved in drug metabolism and activation. It is present in colon, liver, small intestine, heart, brain and testis. The hydrolase activity of small intestine is attributed to carboxylesterase 2 human.[2][3] It has two glycosylation sites at 103 and 267 residues with molecular mass 60kDa.[4]

Application

Carboxylesterase 2 human has been used:

• in the in vitro enzyme-based inhibitor screening assay[5]
• in substrate selectivity assay with emission ratiometric two-photon probe (SE1)[6]
• as a reference standard in the enzyme assay with various 4-nitrophenyl and 1-naphthyl based substrates[7]

Carboxylesterase 2 may play a key role in biotransformation of a variety of ester containing drugs and prodrugs.[3]

Delivers high catalytic activity, ideal for robust high-throughput screening assays including drug-drug interaction studies, and pharmacokinetic studies for evaluating pro-drugs and non-CYP pathways of elimination.

Biochem/physiol Actions

セリンエステラーゼファミリーのメンバーであり、エステルおよびアミド結合を加水分解します。カルボキシエステラーゼ2は、小胞体結合型加水分解酵素であり、エステル含有治療のための生体異物の解毒および活性化において重要な役割を果たします。また、カルボキシエステラーゼ2は、ヘロインやコカインなどの薬物の解毒にも関与します。本酵素は、脂質代謝において役割を担っていると考えられています。

Carboxylesterase 2 human identifies the site containing a large alcohol and small acyl group.[8] It shows less transesterification activity due to presence of conformational interference in active site.[3]

Other Notes

One unit will produce 1 nanomole of 4-nitrophenol from 4-nitrophenyl acetate per minute at pH 7.4 at 37 deg C.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable