D7808
PCR 低塩基対ラダーセット
marker set for electrophoresis of PCR fragments
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この商品について
UNSPSC Code:
12352200
grade
Molecular Biology
form
liquid
usage
50 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
2-8°C
General description
Sigma′s PCR PCR Low Ladder Set contains one vial each of the PCR 100 bp Low Marker and the PCR 20 bp Low Marker. When mixed together, the ladder appears with bands every 20 bp, with every 5th band more intense. About two ul of the mixed ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Sigma′s PCR Low Ladder Set is provided as three separate tubes:
1 vial (25 ug) PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial (25 ug) PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial 6x loading buffer (250 ul volume) containing 15% Ficoll, 0.06% bromophenol blue, 0.06% xylene cyanol FF, and 30 mM EDTA.
1 additional empty tube is provided for storing the mixture.
1 vial (25 ug) PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial (25 ug) PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
1 vial 6x loading buffer (250 ul volume) containing 15% Ficoll, 0.06% bromophenol blue, 0.06% xylene cyanol FF, and 30 mM EDTA.
1 additional empty tube is provided for storing the mixture.
保管分類
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
D7808-1SET-PW: + D7808-1SET: + D7808-VAR: + D7808-BULK:
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