One unit of DNA equals 50 µg. Molecular biology grade TE buffer is suitable for diluting DNA.
D4889
デオキシリボ核酸 ナトリウム塩 from Escherichia coli strain B
Genomic, unsheared
別名:
DNA
ログインで組織・契約価格をご覧ください。
サイズを選択してください
表示を変更する
| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
0.25 unit | カートの在庫状況を確認する | ￥8,010 |
1 unit | 在庫fromsìså¬368 | ￥20,400 |
5 units | カートの在庫状況を確認する | ￥68,600 |
この商品について
CAS番号:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
297-226-7
NACRES:
NA.52
MDL number:
Biological source:
Escherichia coli
Form:
lyophilized powder
Solubility:
water: ~1 g/L
Storage temp.:
2-8°C
￥8,010
カートの在庫状況を確認する
高品質はそのまま、さらにお手頃な価格になりました
biological source
Escherichia coli
Quality Segment
grade
Molecular Biology
form
lyophilized powder
mol wt
>45 kb
solubility
water: ~1 g/L
storage temp.
2-8°C
General description
Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA.
Application
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Biochem/physiol Actions
50%GC;Tm = 90.5°C
Preparation Note
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Analysis Note
DNAの質・完全性の評価は、パルスフィ-ルドゲル電気泳動およびエチジウムブロマイド染色により行っています。DNAには、RNAや制限酵素消化を阻害するおそれのある不純物は含まれません。
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).
1 of 1
当該品目 | |||
|---|---|---|---|
| description Genomic, unsheared | description Type VIII | description - | description aqueous glycerol solution |
| grade Molecular Biology | grade - | grade - | grade - |
| biological source Escherichia coli | biological source - | biological source - | biological source Escherichia coli |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form fibers | form solid | form aqueous glycerol solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −70°C |
| solubility water: ~1 g/L | solubility water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow | solubility H2O: 2 mg/mL, clear to hazy | solubility - |
Still not finding the right product?
Explore all of our products under デオキシリボ核酸 ナトリウム塩 from Escherichia coli strain B
保管分類
11 - Combustible Solids
一体何が起きているの？
WGK 3
引火点 (F)
Not applicable
引火点（°C）
Not applicable
PPE（個人用保護具）
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
D4889-5UN: + D4889-BULK-N: + D4889-.25UN: + D4889-PH: + D4889-VAR: + D4889-BULK: + D4889-1UN: + D4889-.25UN-PW: + D4889-1UN-PW: + D4889PROC: + D4889-5UN-PW:
jan
-
D4889 Sigma-Aldrich Deoxyribonucleic acid sodium salt from Escherichia coli strain B I have 1 Unit of this DNA. Just want to make sure if it =50ug. Also, Can i use TE buffer to dilute the DNA? thank you for your help.
1 回答-
役に立ちましたか？
-
-
what is the absolute amount of DNA in this product is it 50µg?
1 回答-
This product is supplied in a unit size. 1 unit of any DNA = 50 µg.
役に立ちましたか？
-