CRISPR16H

Lenti CRISPR HPRT1 gRNA Control Transduction Particles, High Titer (LV02 vector)

• UNSPSCコード: 41106609
• NACRES: NA.51

特性

• 形状: liquid
• 品質水準: 200
• 包装: vial of 8x25 μL (aliquots)
• 濃度: (5x10⁸ TU/ml (via p24 titering assay))
• アプリケーション: CRISPR
• 輸送温度: dry ice
• 保管温度: −70°C

詳細

詳細

The Lenti CRISPR HPRT1 gRNA Control Transduction Particles (LV02 vector) product is a critical positive control used to monitor transduction efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control uses a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and Ef1a-driven Puromycin resistance casette. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

アプリケーション

Functional Genomics/Screening/Target Validation

その他情報

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

法的情報

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable