AFRC 84B4

Reactivity: Anti-Haemagglutinin, influenza, 94120829

• UNSPSCコード: 41106514

特性

• 由来生物: Horse x Mouse/Horse Hybridoma
• イムノドン: Horse sub-mandibular lymph node
• 骨髄腫: AFRC 17D1
• 反応性: Anti-Haemagglutinin, influenza
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Horse sub-mandibular lymph node x AFRC 17D1

細胞株の説明

The heterohybridoma AFRC 84B4 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 84B4 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

免疫原

Influenza A equine 2 virus

アプリケーション

Haemagglutinin detection

The isotype of the monoclonal antibody produced by this cell line is horse IgG.

培地

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

その他情報

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