RC-4B/C1

94020810, rat brain, Epithelial-like

• UNSPSCコード: 41106514

特性

• product name: RC-4B/C1, 94020810
• 由来生物: rat brain
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Gonadatrophs
• 受容体: Gonadotrophin-releasing hormone receptors
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat pituitary adenoma

細胞株の説明

RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.

アプリケーション

Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.

培地

DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 10% CO₂; 34°C. Saturation density is reached at 1x 100,000 cells/cm². Cells may attach lightly and so handling should be kept to a minimum. Care shoul

その他情報

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