BE10-Late

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSCコード: 41106514

特性

• 由来生物: rat brain
• 包装: tube of 5 μg 93040120-DNA-5UG; pkg of vial of cells 93040120-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Not specified
• 製品: Plasminogen activator activity
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat, BDIX, foetal brain, malignant

細胞株の説明

Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.

アプリケーション

Study of carcinogenesis, tumourigenicity studies

培地

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm² using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C.

その他情報

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