U28

Reactivity: Anti-IgG2a, mouse, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 93022518

• UNSPSCコード: 41106514

特性

• 由来生物: Mouse x Mouse Hybridoma
• 包装: tube of 5 μg 93022518-DNA-5UG; pkg of vial of cells 93022518-1VL
• ImmunoDon: BALB/c mouse spleen
• 骨髄腫: Sp2/0
• 反応性: Anti-IgG2a, mouse
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: IgM
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse Sp2/0 x Mouse BALB/c spleen

細胞株の説明

The hybridoma U28 was obtained by fusing SP2/0 myeloma cells with spleen cells from BALB/c mice that had been given repeated intranasal infection with a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" allotype and the Fc region. There is no cross reactivity with A/PR/8/34 influenza virus, DNP, NP or DNA cytoskeletal proteins. Contains a CH2 domain.

免疫原

A/PR/8/34 mouse pathogenic human influenza virus

アプリケーション

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantification

培地

RPMI 1640 + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

その他情報

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