HT29 gluc C1

92012401, human colon, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: HT29 gluc C1, 92012401
• 由来生物: human colon
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian colon adenocarcinoma

細胞株の説明

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

培地

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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